Camv35s polya
WebGeneral notes. Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow … WebJan 3, 2024 · The reporter beta-glucuronidase (GUS) gene was driven by the CaMV35S promoter and terminated by the CaMV35S polyA signal. Successful transformation was detected using polymerase chain reaction (PCR) technology. Detecting a positive result for the T-DNA hptII gene and a negative result for the intercistronic region between Vir B …
Camv35s polya
Did you know?
Web1. A method of delaying growth of a plant comprising the steps of: a) providing a TPS protein encoded by a plant TPS gene; b) designing a suitable modification to the plant TPS gene by truncating or deleting an N-terminal part of the TPS protein encoded by the plant TPS gene in order to achieve an increased trehalose-6-phosphate synthase activity; c) cloning the … WebCaMV35S PolyA PMI CaMV35S promoter Ubiquitin pro CryIA(b) gene T-Boder(right) nos BamHI Kpnl Xhol Hindlll pUBB-Man EcoRI XhoI XhoI XhoI A B Figure 1. Diagram of the vector pUBC-Man (A) containing the gene cry1Ac and the vector pUBB-Man (A) containing the gene cry1Ab . Both the two vectors contain the
WebMonday - Friday, EST. 10:00AM - 5:00PM My Account Order Status Wish Lists Sign in Create an Account WebCaMV35S polyA It’s a kind of terminator derived from cauliflower mosaic virus (JD Williamson et al 1989). References John D. Williamson, Marlaya E. Hirsch-Wyncott, …
WebNov 25, 2015 · 由此得出，CaMV35S启动PtrpC同样可oxysporum中起作用。 然而，本研究同样比较了带有PtrpCCaMV35SpCAMBIA一1300二者转差别很大，前者的转化效率可达到385-460个转化子／10绿木霉分生孢子，而后者转化效率仅为20-50转化子／10绿木霉分生孢子，仅为前者的1／10。 WebJan 1, 2024 · The of sesterterpene molecules containing a C25 skeleton is catalyzed by chimeric enzymes that carry out both chain elongation and terpene cyclization reactions synthase enzymes, together with other related enzymes such as diterpene and farnesyl diphosphate synthases, in a single fungal strain.
WebFeb 24, 2024 · pCAMBIA plant transformation vector. (A): an empty T-DNA construct hosting a neomycin phosphotransferase (nptII) gene driven by a CaMV35S promoter and terminated by a CaMV35S polyA; (B): same as ‘A’ except that the construct harbored a hygromycin phosphotransferase (hptII) gene. Figure 2:Half-strength (0.5X) MS-agar …
WebCaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilised metal affinity chromatography resins. Designed for promoter testing in planta, this vector features a promoterless version of gusA imperial house condoWebThe present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the m imperial house 8 kean st london wc2b 4asWebMay 6, 2015 · The CaMV35S polyA module in the plasmid pCAMBIA-1300-221 was selected as the terminator for the basta-resistance cassette, and the entire sequence from the first base immediately after the hygromycin module to the unique SacII restriction site of the plasmid pCAMBIA-1300-221 was amplified with the primer pair CaMV35S-5 and … litchfield national park excursionsWebClone hptII gene into pMD18T to produce pUC18-CaMV35S-hptII-CaMV35S polyA (Table 4) and checked by sequencing (Fig. 5 F). 3. Amplify upstream fragment of Au3446 … litchfield myWebby a CaMV35S promoter and terminated by a CaMV35S polyA; (B): same as ‘A’ except that the construct harbored a hygromycin phosphotransferase (hptII) gene. Figure 2. Half-strength (0.5X) MS-agar plates for selection of transgenic Arabidopsis thaliana L. plants. Plate A: wild type (WT) imperial house condo miami beachWebCaMV35S polyA Figure 1. Schematic representation of the T-DNA region of pCAM BIA2301. The plasmid contains a catalase intron in the GUS-coding sequence. The CaMV 35S and NOS terminator drive the GUS reporter gene. The selective marker gene NPT II is driven by CaMV35S promoter and terminated by the CaMV35S poly A signal.? Springer litchfield municipality mapWebApr 7, 2024 · Watermelon (Citrullus lanatus) is an economically important cucurbit crop. Its pulp is rich in antioxidant carotenoids, which confer a variety of flesh colors. ClPsy1 (Phytoene Synthase) is the rate-limiting enzyme for carotenoid synthesis; however, the promoter activity of ClPsy1 is still unknown. In the present study, promoter sequences … imperial house banqueting