Biotin 488
WebIt is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 … WebAnti-Rabbit Alexa Fluor® 488 secondary antibody validated for ICC/IF, IHC-Fr, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 1396 publications. ... Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ...
Biotin 488
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WebMay 16, 2024 · HABA is displaced when biotin binds to the Alexa Fluor 488 dye-labeled avidin, resulting in decreased FRET efficiency. This mechanism results in an increase in fluorescence intensity directly related to the amount of biotin present in the sample. The assay is able to detect as little as 4 pmol biotin in a 0.1 mL volume within 15 min of … WebAlexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. …
WebIn the absence of biotin, HABA quenches the fluorescence emission of the Alexa Fluor 488 dyes via FRET HABA is displaced when biotin binds to the Alexa Fluor 488 dye-labeled avidin, resulting in decreased FRET efficiency. This mechanism results in an increase in fluorescence intensity directly related to the amount of biotin present in the sample. http://www.rx-8.com/chanpinzhongxin/202479.html
http://www.nanocs.net/Alexa-fluor488-PEG-biotin-3k.htm WebCompare Alexa Fluor ® 488 to FITC. Western blot. Secondaries optimized for chemiluminescence. Infrared fluorescent western blot. WB protocol. Learn about biotin-labeled antibodies, HRP, and fluorescent secondary antibodies so you can choose the correct secondary antibody for your application.
WebThe conjugates of streptavidin are commonly used together with a biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other biomolecules in western blots, flow cytometry, imaging and microscopy, and microplate assays. XFD488-streptavidin conjugate is equivalent to Alexa Fluor® 488 streptavidin ...
WebLabeling via Click-iT technology with a biotin moiety followed by biotin labeling with streptavidin-peroxidase; Upon the addition of a chromagen substrate, a dark brown signal results ... Cell proliferation analysis using … clifton waters retirement villageWebBlue Laser (488 nm) Application Notes. Streptavidin-Alexa Fluor® 488 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with … boats for sale america usaWebMar 9, 2024 · Besides, we confirmed the combination of OPBA-PEG-biotin and biotinylated anti-EpCAM antibody on the MNPs using the confocal laser scanning microscopy (CLSM). On one hand, 1 mg MNP@PDA@GO@OPBA-PEG-biotin and 1 mg MNP@PDA@GO were mixed with 80 μg SA-FITC, respectively, and left to react at room temperature for 1 … clifton waters villageWebAtto 488-Biotin BioReagent, suitable for fluorescence, ≥90.0% (HPLC); find Sigma-Aldrich-30574 MSDS, related peer-reviewed papers, technical documents, similar products & … clifton watt md berkeleyWebThe City of Fawn Creek is located in the State of Kansas. Find directions to Fawn Creek, browse local businesses, landmarks, get current traffic estimates, road conditions, and … clifton waters retirement village bairnsdaleWebAPDye™ 488 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor … boats for sale anchorage alaskaWebAlexa fluor 488 labeled PEG Biotin (AF488 PEG Biotin is a green fluorescent PE biotin derivative having excitation/emission wavelength around 495 nm/520 nm. Alexa fluor 488 … clifton waterstones